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The first step of the lab is to prepare the needle and syringe that will be taking the hemolymph. The syringe size was 1 ml, and the intention is to collect between 0.5 and 1.0 ml of hemolymph. The needle size was 20 gauge, because anything smaller would destroy the hemolymph cells. Then the lobster was picked up with a firm grim around the dorsal celphao-thorax region and flipped over to expose the ventral side. The hemolymph was be extracted from the central midline of the ventral pre-branchial region, of the first section. Although, before piercing the membrane, the bevel of the needle had to be pointing up...
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