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This will allow the betalain to leak out from the cell vacuole. Under extreme cold the cell membrane will freeze and fracture also allowing the betalain to leak out into the extracellular.
The Betalains function is really unknown, but it has been suggested that when present in flowers it is used to attract insects for pollination and when present in seeds attracts birds for dispersion. There is no indication that betalains protect plants from pathogens or herbivores, and they also do not absorb UV light. Betalains are also poor pH indicators.
In this experiment the betalain will be measured by using a colorimeter...
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