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Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by Edwin Southern to a technique he developed for DNA detection.
Western blot analysis has the ability to detect one specific protein in a mixture of any number of proteins while at the same time giving you information about the size of the protein. It does not matter whether the protein has been synthesized in vivo or in vitro. However, this method does require the use of a high-quality antibody directed against a desired protein. During western blotting procedures, generally, proteins are solubilized with detergents and reducing agents, and then are separated in polyacrylamide gels...
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