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Not all of these tests were performed on every culture, as some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows:
Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium, the bottom of the BHIA dish was labeled with the bacterium number, initials, and section; then divided into four quadrants. The second step, we used the septic technique to transfer a small amount of culture with a flame-sterilized inoculating loop to the first quadrant, flamed and cooled the loop again then transferred a small amount of the culture from the first quadrant to the second using the quadrant streaking method as illustrated on page 18 of the lab manual, repeating this process until all four quadrants were properly streaked...
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