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It also must have a weak cell wall and plasma membrane. The cell then should undergo rapid binary fission. The foreign DNA is transferred to the competent cell via a vector. The primary vector used is the plasmid. Plasmids are extra chromosomal and contain the gene of interest, in this case bioluminescence. The R plasmid, which causes the resistance to Ampicillin, confirms antibiotic resistance. It makes it able to identify which cells under went transformation. Restriction endonucleases can be used to cut and insert pieces of foreign DNA into the plasmid. Once the bacteria or competent cell takes up the plasmid, the restriction enzymes in the host cell immediately begin cutting up the foreign DNA...
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