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In past research, progesterone has exhibited harmful effects on aquatic organisms, so being able to quantify the concentration in a sample will allow for a better understanding of the steroid's toxicity levels. In the SPE method being used, the analytes are retained in the sorbent bed and the matrix is washed away. The sorbent bed is hydrophobic and made of octadecylsiloxane, which is preferred for the extraction of hydrophobic steroids. Once the sample has made its way through the sorbent bed the analytes can be collected by using various washing solutions and eluting the solution. Solid phase extraction is an extremely useful sample preparation technique because it limits many problems that arise from the use of liquid-liquid extraction...
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