Step-Wise Purification and Characterization of a Lactate Dehydrogenase Isozyme from unknown tissue sample of Sus domestica
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Title: Step-Wise Purification and Characterization of a Lactate Dehydrogenase Isozyme from unknown tissue sample of Sus domestica

Essay Details:

Subject: Science
Author: Ambrose A
Date: April 25, 2015
Level: University
Grade: B
Length: 6 / 1539

Essay text:

We concluded our sample was from the heart of Sus Domestica. The LDH tetramer found in the heart tissue consists of 4 H isozyme subunits of protein coded by the LDHA gene. 4 different steps of purification were attempted and samples taken at the conclusion of each major step were tested for activity. It was found that the 65% cut pellet, the second intermediate, had the highest LDH activity of all purifications. Our SEC purified product was also highly active but second to the 65% CP. 2) Abstract. This should be a brief summary of the major conclusions from your experiments, and should not be more than a paragraph in length...

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