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A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp.
Finally, a Mannitol Salt Agar was streaked and incubated at 37 degrees Celsius for 24 hours to determine whether the bacteria was Staphyloccus aureus or Staphylococcus epidermidis. A positive conformation of Staphylococcus aureus is the ability of the bacteria to ferment the carbohydrate mannitol which produces yellow growth when mannitol is b roke down to lactic acid.
After 24 hours, the bacteria colonized and yielded a yellow growth validating that it was unquestionably Staphylococcus aureus...
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