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Samples: pharyngeal and nasal exudates, nasal and pharyngeal washing, necroptic fragments
2. Influenza antigens may be demonstrated within the epithelial cells using fluorescent antibodies.
3. Isolation: on embrionated egg (in amniotic or alantoidian liquid) or cell culture
4. Identification is made mainly by hemagglutinoinhibation with specific serum
using cock (rooster) erythrocytes.
Other techniques for identification: complement fixation
II. Serologic tests
Are based on the at least four-fold rising of antibodies titer in the serum collected in convalescence.
Hemagglutinoinhibation (HIA)
-place diluted serum in a plate with wells
-place standard antigens (A or B from one of the circulating variants N1H1, N1H2, N1H3, N2H1, N2H2, N2H3)
-incubate for 60 minutes
-place a suspension of cock erythrocytes
-incubate for 1 hour and read
The principle of the reaction:
Influenza viruses have hemagglutinin antigens (H) so that if a suspension of viral antigen is placed in contact with erythrocytes the erythrocytes will be agglutinated...
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