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I observed the broth for growth characteristics. I characterized the surface as membranous because there was a thin film of growth at the top. The subsurface I characterized as turbid because it was very cloudy. After agitating the tube, I was able to characterize the sediment as viscid.
e. I recorded the broth growth characteristics onto my Descriptive Chart. (Brown, pg 226)
i. Surface: Membranous
ii. Subsurface: Turbid
iii. Sediment: Viscid
Four quadrant streak
f. I rolled my broth tube to get a good dispersion of the organism.
g. Next, using four quadrant streak techniques for isolated colonies, I stroked my TSA plate...
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