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Proteomics Investigation Of Sample Loading

Proteomics: Investigation of sample loading techniques for Iso-Electric Focusing

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Title: Proteomics: Investigation of sample loading techniques for Iso-Electric Focusing

Essay Details:

Subject: Science

Author: Rebecca V

Date: June 26, 2014

Level: University

Grade: A

Length: 15 / 4362

Essay text:

An iso-electric point is the specific pH at which the net charge of a protein is zero. 1 After this, further separation is carried out, whereby proteins are separated according to their molecular weight. 1 Abstract To identify the various types of proteins that are present in a protein sample, the proteins must first be separated. The proteins are separated by first dimension isoelectric focusing (IEF), followed by second dimension sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The protein sample is loaded onto an immobilised pH gradient (IPG) strip. IEF, an electrophoretic method, is then carried out...

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