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Then a mark was placed on the outside of the flask to indecate the level of the solution before heating. The flask opening had perafilm placed over it so that there was little to no evaporation. The solution was then placed in the microwave and heated. The solution was then heated for one min and swriled for evenly dissolved
agarose. The agarose was then cooled, so that it was not to hot and the plate would crack. Some water was added to the solution because of there was some evaporation during heating. Once the gel had cooled , it was poured into the plate between the rubber dams. The plate was filled about half way up the comb arms...
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