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They are neither used up in the reaction nor do they appear as reaction products.
The rate of a reaction increases until all the active site of an enzyme is filled with a substrate or the reaction has reached its maximum rate, or stopped.
The basic enzymatic reaction can be written as follows:
S + E > P + E
SUBSTRATE ENZYME PRODUCT ENZYME
As you can see, when an enzyme is added to a reaction, it reacts with the substrate but also comes back out at the end, it never changes.
The lock and key theory utilizes the concept of an "active site." The concept holds that one particular portion of the enzyme surface has a strong affinity for the substrate...
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