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In prokaryotes that perform photosynthesis, there are no chloroplasts; instead, photosynthesis occurs in association with the plasma membrane or infoldings of the membranes. Chloroplasts are found mainly in the cells of mesophyll, the green tissue in the interior of the leaf.
In the lab we replaced the electron-accepting coenzyme, NADP, with the blue DPIP dye. When DPIP was reduced, it had changed its color from blue to colorless. The DPIP reduction led to the decrease in light absorbance measured by the colorimeter.
Procedures
Plant Pigment Chromatography
1. Obtain and wear safety goggles and a lab apron...
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