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Then, the seed is transferred to fermentor. These fermentors are operated in fed-batch mode under standard conditions in order to optimize the synthesis of penicillin. After that, the product is withdrawn by solvent extraction in the downstream.
Inoculum development
The first step in the fermentation process is inoculation of cultures. The main purpose of this and subsequent inoculum development steps is to increase the concentration of fungal mycelium (biomass) to give a population which can be added to the next step to assure that each step will be reasonably short and the large-scale equipment is used efficiently...
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