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We tied off one end of the dialysis tubing to create a bag, filled it with distilled water, and tie of other end to close. The bag is carefully blotted to remove any water spilled during the filling and then weighed and recorded for an initial mass. Then we filled a 250 ml beaker with only distilled water. Immerse the bag in the beaker of distilled water and let stand for 30 minutes. After 30 minutes we removed the bag from beaker and carefully blot excess water to determine final mass. No difference in mass shows that no passage of water through dialysis membrane resulting in an isotonic solution...
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