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Trim each cylinder to 15-mm in length.
*Place these cylinders of beet tissues in a beaker and run tap water over then for 2 minutes to wash betacyanin from the injured cells on the surface.
*Discard the slightly coloured solution, and place the 4 beets sections into 4 dry test tubes.
*Keep all beets at room temperature
for 20 minutes and shake them occasionally. After 20 minutes, remove and discard the beet sections and observe the extent of membrane injury according to the amount of betacyanin that diffused into the organic solvent.
*Quantify the relative colour of each solution between 0 (colourless) and 10(darkest red)...
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