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Separation occurs by causing the larger particles to separate to the bottom as a particle or pellet, and the smaller will remain suspended in the liquid, or supernatant. Due to smaller particles separating out first, centrifugation is often done in small steps so that the supernatant takes off all larger unwanted cells first.
The basic process of centrifugation:
1) The tissue must be homogenized to allow it to separate.
2) A salt solution is added to the sample and together they are placed in the centrifuge.
3) The centrifuge is run once for every group of cellular bodies that need to be separated and the pellet is removed...
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