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Essay Details:
Essay text:
The spectrophotometer includes a
light bulb, a reflector, and a detector. When a sample is in place and
the chamber lid is closed, light from the light bulb passes through
it. The detector measures the amount of light transmitted. The white
light from the bulb is separated into different wavelengths by
reflecting the beam off of a diffraction grating, forming a spectrum.
A mirror is used to control the angle at which the light is reflected,
so light of a single wavelength can be passed through the sample. A
pigment solution (such as spinach and acetone) is exposed to different
wavelengths of light to determine the points of the highest absorbance
and transmittance percentages...
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