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Using PCR, a selected target DNA can be amplified several million-fold in just a few hours. Within a dividing cell, DNA replication involves a series of enzyme-mediated reactions, whose end result is a faithful copy of the entire genome. Within a test tube, PCR uses just one indispensable enzyme-DNA polymerase-to amplify a specific segment of the genome.
During cellular DNA replication, enzymes first unwind and separate, or denature, the DNA double helix into single strands. Then, the enzyme RNA polymerase synthesizes a short stretch of RNA complementary to one of the DNA strands at the start site of replication...
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