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Then the 6 – carbon molecule is split in half with 3 on each side with 1 phosphate attached.
In the second half of glycolysis, an additional inorganic phosphate is added into the 3 – carbon phosphate. The additional phosphate and the NAD+ come together and make NADH. Then it goes into a transfer, which is a reduction of ADP to ATP twice which comes out with a 3 – carbon pyruvate. ADP is converted to ATP through substrate level phosphorlyation. Which is 1 NADH and 2 ATP molecules. 4 ATP (2 ATP from each side) – 2 ATP (from the first step) = 2 ATP (the net yield). For glycolysis to continue NADH has to be recycled to NAD+ by either fermentation or aerobic respiration...
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