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Essay Details:
Essay text:
This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE).
Purpose: To illustrate the process of transformation and perform it using Green Fluorescent Protein.
Experimental Design: This experiment will achieve its purpose by allowing the student to perform a transformation following step by step instructions.
Procedure:
Laboratory Procedure for pGREEN Steps
1. Mark the sterile 15mL tubes with the correct labelings. (LB+plasmid, LB/Amp+plasmid).
2. Use a sterile transfer pipet to add 250mL of ice cold calcium chloride to each tube...
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