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This type of ends are referred to as "Blunt Ends"
Ex: AlvI cuts right in the middle of its sequence AGCT
Two types of gels are used for DNA analysis
(1) Agarose gel:
A) Derived from seaweed
B) Used for analyzing long DNA chains 500 ? 20,000 base pairs long
(2) Acrylamide Gel:
A) Good resolving power
B) Used for analyzing short DNA chains 1- 500 base pairs
t
Mapping the recognition sites for the two restrictions sites relative to each other
(1) You have double stranded DNA of a particular length (1 unit).
(2) You know that Enzyme I cuts this fragment as shown above.
(3) When Enzyme II was used to cut this DNA, you obtained two
fragments having 0...
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