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In addition to separating specific proteins and DNA fragments of different sizes, electrophoresis techniques are frequently used to determine:
?? The length of DNA fragments, in the base pairs
?? The molecular weight of specific proteins
DNA Fingerprinting
From person to person, DNA is basically the same. But scientists have identified regions along the human DNA sequence where the order of As, Ts, Gs and Cs varies considerably. DNA profiling takes advantage of this variability. With the exception of identical twins, every person's DNA is unique.
?? Restriction Fragment Length Polymorphisms (RFLPs) are produced when DNA samples are cut with REs
?? Number and position of fragments run on the gel is unique for each individual
?? Identification:
?? Criminal cases
?? Paternity testing
?? Identification of missing persons
?? Genetic Disease Diagnosis
Human Genome Project
?? Effort to map the entire
human genome?K
?? Four approaches:
?? Genetic (linkage) mapping
?? Frequency in which genes are inherited together
?? Physical mapping
?? Determination of the order of genes on the chromosome
?? DNA Sequencing
?? Determination of the order of nucleotides
?? Analyzing the genomes of other species (compare)
?? E...
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