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He had placed the bottom half of a dissected frog near a plate-type electrical machine. When his assistant touched the frog’s sciatic nerve with a metal scalpel, they observed sparks in the machine as well as movement in the frog’s leg’s almost, as if they were alive. Galvani was puzzled by how they occurred simultaneously. In one of his essays, he wrote "While one of those who were assisting me touched lightly, and by chance, the point of his scalpel to the internal crural nerves of the frog, suddenly all the muscles of its limbs were seen to be so contracted that they seemed to have fallen into tonic convulsions...
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