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This study (Nyholm and Mcfall-Ngai, 1998), examines this diel rhythm in terms of the cycle and the contents of the matrix in the light-organ of E. scolopes, "including aspects of nutrient exchange, host immune response, and the control of symbiont number"(Nyholm and Mcfall-Ngai, 1998).
Under a natural 12 hour dark 12 hour light cycle, the animals were anesthetized in ethanol and the light-organ was exposed to a light source and after staining the host cells, the exuded material was collected (lack of a light stimulus produced no venting). However, the matrix obtained from the organ was prepared for observance under scanning and transmission microscopy at different time intervals in the cycle...
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