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Without the unionized -COOH group of Glu-35 and the ionized -COO- of Asp-52, the catalytic action of lysozyme would cease.
Hydrogen bonds are easily disrupted by increasing temperature. This, in turn, may disrupt the shape of the enzyme so that its affinity for its substrate diminishes. The ascending portion of the temperature curve red arrow in right-hand graph above reflects the general effect of increasing temperature on the rate of chemical reactions graph at left. The descending portion of the curve above blue arrow reflects the loss of catalytic activity as the enzyme molecules become denatured at high temperatures...
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