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Ezrin Silencing By Small Hairpin Rna Reverses

Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.

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Title: Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.

Essay Details:

Subject: Science

Author: Crystal S

Date: April 17, 2016

Level: University

Grade: A

Length: 3 / 775

Essay text:

There were four types of human breast cancer cell lines that were obtained; MCF-7, MDA-MB-453, MDA-MB-435s and MDA-MB-231. These breast cancer cell lines were then tested, using Western blot analysis, for the expression of ezrin and the results were MDA-MB-231 contained the highest expression of ezrin and MCF-7 was the lowest in the expression of ezrin. The cell line selected for this study was MDA-MB-231. The type of RNA interference that was used was ezrin small hairpin RNAs (ezrin shRNAs). Ezrin shRNAs is a silencing gene that was used to silence the protein ezrin from its functions, which in this case it would be metastasis...

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