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Essay text:
Prosthetic groups and co-enzymes
WHAT IS A CO-FACTOR?
? Some aspect over and above the enzyme which is needed for the enzyme to work
? In some cases: enzyme + co-factor are needed for enzyme action.
INORGANIC IONS
Around the enzymes helps do its job
? They mainly influence shape of enzyme molecule e.g. Cl? is needed for salivary amylase. Many trace elements can fulfil its role e.g. Zinc, Copper.
PROSTHETIC GROUPS
? Similar to co-enzyme
? Usually act as carriers during enzyme controlled metabolic change
CO-ENZYMES
? Very similar but loosely attached to the enzyme molecule
TEMPERTURE
? If temperature goes above a certain level the vibration will break some of the bonds that hold the enzyme
? Active site changes so no longer fit
? At this enzyme denatures
GRAPH OF RATE OF REACTION
The steep declines after an optimum
? At low temperatures, enzymes are inactivated rather then denatured ( the extent is dependent on time)
? In enzyme experiments, remember technique of equilibration i...
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