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Enzyme activity is important in this reaction because enzymes act as catalysts, they decrease the activation energy (the difference between the transition state and the energy of the reactants or products) needed to start the reaction and therefore speed up the reaction rate. A substrate molecule must bind to the enzyme at the active site before the substrate can be catalyzed. The rate at which an enzyme can catalyze the reaction is affected by factors such as enzyme concentration, substrate concentration, the affinity of the enzyme for the substrate (how well the enzyme binds to the substrate), concentrations of cofactors and coenzymes, pH and temperature...
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