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At a pH of 10, EDTA reacts with calcium ions, which bind to any of the six sites. A pH of 10 is required for Ca2+ ions to bind with EDTA, because at a lower or higher pH, the Ca2+ ions have to compete with hydrogen or hydroxide atoms for binding sites. Eriochrome Black T was the indicator used during the titration, which turns from wine-red to blue when the equivalence point is reached. The EDTA needed to be standardized, because it is a secondary standard, due to its instability problems prohibiting it to be obtained with a 99.9% composition. Due to this, a primary standard, CaCO3 is reacted with EDTA to standardize the EDTA in solution...
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