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Messenger RNA from the cells of interest is first converted into cDNA which is then labeled with a fluorescent probe and incubated with the microarray where hybridization occurs; positions of hybridization are detected with a scanning-laser microscope (1). For comparison studies, typically two differently labeled samples (a test and a control) are mixed and the scanned intensity of each dye is proportional to the amount of hybridization by the respective cDNA (4).
Regardless of simplicity, what makes microarrays truly ground-breaking is its ability to analyze thousands of nucleotides in a single assay as opposed to blotting techniques (5)...
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