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Essay Details:
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Essay text:
This lab is solely focused on the concentration of sucrose in a potato in relationship to the rate of diffusion.
Experimental Design
Equipment list:
3 15 cm lengths of dialysis tubing
3 400 mL beakers
1% NaCl solution, 10 mL
5% NaCl solution, 10 mL
10% NaCl solution; 10 mL
1 potato with 4 plugs taken out (approx 3cm each)
0.0M Sucrose, 5-10 mL
.33M Sucrose, 5-10 mL
.67M Sucrose, 5-10 mL
1.0M Sucrose, 5-10 mL
1 microscope slide
1 Coverslip
Scalpel
Forceps
Compound Microscope
LabPro
Computer with Logger Pro installed
Vernier Conductivity Probe
Vernier Gas Pressure Sensor
1 Graduated Cylinder; 10 mL
1 Funnel
1 Craft stick
1 Pipet, 1 mL
1 Thermometer
Plastic wrap
1 Onion
1 Cork Borer
Distilled Water
1 Balance
Paper Towels
1 Wash Bottle with dH2O
1 400 mL beaker for waste liquid
1 Beaker for dH2O
Procedure:
1) Open Lab Pro and set the conductivity probe for 2000 uS/cm
2) Get 3 400 mL beakers filled with 300 mL of dH2O; label 1%,5%, and 10%
3) Dry a piece of dialysis tubing and fill with 10 mL of 1% NaCl solution (repeat with 5% and 10%)
4) Place the tubing in the corresponding beaker and stir slowly for approximately 15 seconds
5) After stirring start your data collection let it run for 2 minutes
6) Use LabPro to calculate the rate of diffusion using the line function
7) Clean the area before proceeding to part 2
Part 2
1) Set up your Gas Pressure Sensor by connect it to channel 1 of the LabPro
2) Open the preset data collection table called "22 osmosis"
3) Cut a piece of string that when tied will still fit around your potato
4) Use a cork borer to make 4 well spaced hole in the potato 3 cm down
5) Save the plugs and mark the holes (0...
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