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Add one single drop of the crystal violet dye solution, invert to mix the contents of the test tube, and carefully place the tube into the instrument. When the drop hits the surface is your “time zero”, and one minute later you will begin taking readings of %T. Leave the sample tube in the instrument throughout the data collection process. Obtain readings of %T every minute for at least 15 minutes. Record %T values to one decimal place, as this allows you to convert to absorbance and retain three significant figures.
When the 15th and final reading has been taken, remove the sample from the instrument and prepare to re-zero the instrument with a new NaOH blank in another test tube...
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