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The following types of cloning technologies will be discussed: (1) recombinant DNA technology or DNA cloning, and (2) reproductive cloning.
Recombinant DNA Technology
The term recombinant DNA technology refers to the transfer of DNA from one organism to a self replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. Plasmids are self-replicating extra-chromosomal circular DNA molecules and are used to copy genes and other pieces of chromosomes to generate enough identical material for further study. To clone a gene, a DNA fragment containing the gene is isolated using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzyme...
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