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Therefore to understand their function other techniques must be used to study them. The most useful approach that has been determined to analyze these proteins is to use two, site-directed mutagenesis and electrophysiology. This approach has been improved by nonsense suppression methodology. With this a tethered agonist approach was used to map the agonist binding site of ligand-gated channels. This would provide invaluable information for understanding its response to pharmaceuticals and other chemicals in the human system. Previous studies indicated that sub-units near the binding site of the nicotinic acetylcholine receptor may contribute to binding and may actually be the binding sites...
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