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Our negative control will be glass beads to measure to increase or decrease in atmospheric pressure or temperature changes. There is a direct relationship between oxygen consumption and Carbon Dioxide produced, therefore the more O2 consumed the more CO2 produced. To keep the amount of CO2 produced from canceling out any pressure gained or lost from the consumption of CO2 we are adding KOH(Potassium Hydroxide) to the respirometers. Since the CO2 will be selectively removed the change in the volume of gas in the respirometer should be directly related to the oxygen consumed. In this experiment the germinating peas, in both water baths, should consume much more oxygen than the non-germinating peas or the glass beads, because germination has began thus accelerating cellular reactions and the rate of respiration...
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