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Revised November 14, 2005. Accepted November 14, 2005.
The trans-activation response (TAR) RNA stem–loop that occurs at the 5' end of HIV RNA transcripts is an important antiviral target and is the site of interaction of the HIV-1 Tat protein together with host cellular factors. Oligonucleotides and their analogues targeted to TAR are potential antiviral candidates. We have investigated a range of cell penetrating peptide (CPP) conjugates of a 16mer peptide nucleic acid (PNA) analogue targeted to the apical stem–loop of TAR and show that disulfide-linked PNA conjugates of two types of CPP (Transportan or a novel chimeric peptide R6-Penetratin) exhibit dose-dependent inhibition of Tat-dependent trans-activation in a HeLa cell assay when incubated for 24 h...
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Common topics in this essay:
- Regulate Use of Cell Phones on the Road
- biology cells
- Nucleic Acids
- Stem Cell Technology:The Controversies surrounding Stem Cell Research
- Biology Cell Parts
- Biology Laboratory
- Biology: cell functions
- Cell-Penetrating Peptide Conjugates Of Peptide Nucleic Acids (Pna) As Inhibitors Of Hiv-1 Tat-Dependent Trans-Activation In Cells
- Cells And Cell Theory
- Evolution and Its Place In the Biology Classroom
- Solid Phase Peptide Synthesis (SPPS)
- Stem Cells: The holy grail of medical research
- The Greatest Medical Breakthrough?the use of Pluripotent Stem Cells
- A Call to Action: Regulate Use of Cell Phones on the Road
- molecular biology review
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