Biotechnology

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Title: Biotechnology

Essay Details:

Subject: Science

Author: Anna H

Date: July 22, 2016

Level: University

Grade: A

Length: 5 / 1254

Essay text:

After the restriction endonuclease binds, it starts to disrupt, using hydrolysis, the phosphodiester bonds between neighbor nucleotides, causing the H-bonds between base pairs in the cutting region to be broken. This cuts the original double-stranded DNA strand, producing two DNA fragments, which may differ for different restriction endonucleases, depending on where the phosphodiester bond is broken when cut by the endonuclease. This process can produce either blunt ends (where ends of the DNA fragment are fully paired with no overhangs), or sticky ends (where both DNA fragments have nucleotides lacking complementary bases and overhangs are produced)...

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