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I decided to observe 20?, 30?, 40?, 50?, 60?, 70? and 80?.
3. Next, I label each test tube with the temperature to be investigated and then added 2.5cm3 of Trypsin solution.
4. I ensured that I cut the necessary pieces of film using a ruler and a pair of scissors. I cut pieces that were 2cm in length, and 1.6cm in width each.
5. The only water baths of my choice that were running were at 30? and 60?. I therefore had to use the Bunsen burner etc. for the rest of the temperatures (excluding 20?). I kept each test tube heated for 5 minutes.
6. I added the photographic film pieces just before I placed the test tubes in the water baths/ Bunsen burners, and ensured that they submerged fully before I began timing...
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