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Handle the TLC paper with care to prevent oils from your hands to get on the paper. Making the spots small prevent the spots from going in the paths of the other spots on the TLC paper. The TLC paper is placed in the developing chamber to allow the spots to develop. Then they are placed under a UV lamp to observe the spots that formed on the TLC paper. Then in millimeters the spots are measured relative to the solvent front. The iodine analysis is not preformed until after all the UV comparison is complete.
A major deviation was not being able to make the capillary micropipettes.
With spot plat 1, in acetaminophen there are two compounds the second being more polar than the first, the first compound has an Rf of 0...
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