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Extraction Method
Extraction:
Take a 150 mL Erlenmeyer flask, and add to it 8 g of coffee grounds.
Add to it 50 mL of methylene chloride.
Allow the coffee to extract for 5 minutes.
Remove the bulk coffee grounds from the solution.
If coffee is allowed to remain longer, caffeine may become overrepresented in the sample.
Some methylene chloride will be retained by the coffee ?C this is an acceptable loss, and no dilution is necessary.
Preparation:
Separate the coffee extract from above into three equal portions; assuming some loss of methylene chloride, as above, these portions will be about 15 mL each...
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