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R. nigricans was subjected to varying types of carbon sources, Glucose monohydrate, Molasses, Starch and Lactose and at different concentrations, 2% and 4%. The different Nitrogen sources were Corn Steep Liquor 2.0%, Diammonium Hydrogen Phosphate (NH4)2HPO4 .2% , Urea .1%.
Variation of the media was done to investigate what affects the growth of R. nigricans.
MATERIALS AND METHODS
Mediums: 12 Mediums were prepared as shown in Table 1, labeled A-L with varying concentrations of Carbon Source and Nitrogen Source. (Table 1). Mediums were divided into 2 flasks, volumes of 250mL, pH adjusted to 5...
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