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These specific Bacillus spores are used because they are more resistant, and present in greater numbers than are the common microbial contaminants found on patient care equipment. If it is proven that these spores have been killed, it is strongly implied that other potential pathogens in the load have also been killed.
In this method, living spores which are resistant to whichever sterilizing agent is being tested are prepared in either a self contained system, such as dry spore strips, or in sealed vials or ampules of spores in suspension. Then the prepared living spores are exposed to the sterilant...
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