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There must also be an indicator substances to determine the change that took place. Then there must be a tool to measure the change. In this lab a spectrophotometer was used. The ABS value is the actual value and it is used to determine the rate of change.
3. The higher the concentration of the enzyme the more there are to catalyze the reaction. Taking information from graph 1 (change in mL of enzyme), the more mL of enzymes that there are the faster the reaction rate is. It would increase until there was no substrate left available for a reaction.
4. At 5'C the reaction was slow because molecule movement gets slower when the temperature is lowered...
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