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One method of researching this was through gas chromatography/mass spectroscopy (GC/MS) to find nicotine residue compounds in old tobacco pipes. The Boucher site, located in Vermont, had been studied in an effort to find pipes that contain residue from tobacco and from there, determine a date in which it was used. The pipes themselves are not available to be researched, but the residue that was once inside of them is.
Once the residue has been obtained, the nicotine is separated from the substance through a very careful process. "This is done by refluxing an organic solvent, methylene chloride, through a soxhlet extractor which percolates the solvent through the sample" (Rafferty, 2)...
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