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Because the hydrogen peroxide is converting to water and oxygen gas. The bubbles contain oxygen gas. If I had boiled the enzyme solution before adding it to the peroxide, I won’t see the bubbles because temperature too high would denature the enzyme so the hydrogen peroxide cannot convert to water and oxygen. Therefore, there won’t be bubbles in during the reaction.
2. Because as the enzyme reaction time takes longer, there would be less h2o2 in the solution. The h2o2 had converted to water and peroxide. The color intensity become darker as the enzyme reaction time takes longer. This means that the h2o2 concentration in the solution has become less as the time goes by...
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